Chitosan, sodium alginate and gel of Aloe vera (Aloe barbadensis Miller) were employed for the preparation of polyelectrolyte complexes at pH 4 and 6. FT-IR spectroscopy analysis showed evidence on complexes formation and incorporation of the Aloe vera gel. The ζ potential determination of the polyelectrolyte complexes revealed the presence of surface charges in the range of -20 to -24 mV, which results in stable systems. The dynamic moduli exhibited a high dependence on angular frequency, which is commonly found in solutions of macromolecules. The materials showed human fibroblast and lymphocyte viabilities up to 90% in agreement with null cytotoxicity. The polyelectrolyte complexes at pH 6 with Ca2+ were stable, showed high water absorption, satisfactory morphology, pore size and rigidity, characteristics that allowed significant human fibroblast migration in wound closure in vitro assays.
Keywords: (3-(4,5-Dimethyl-thiazol-2-yl)-2-5-diphenyltetrazolium bromide (PubChem CID: 64965); Aloe vera; Calcein-AM (PubChem CID:4126474); Calcofluor white (PubChem CID: 6108780); Cell viability; Chitosan; Chitosan (PubChem CID:71853); Dimethyl sulfoxide (PubChem CID: 679); Ethidium homodimer (PubChem CID: 12328897); Fibroblast; Isopropyl alcohol (PubChem CID: 3776); Lymphocyte; Polyelectrolyte complex; Sodium alginate (PubChem CID: 91666323).
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