Thermosensitive and photocrosslinkable hydroxypropyl chitin-based hydrogels for biomedical applications

Abstract

In situ forming injectable hydrogels based on thermosensitive polymers are being investigated for tissue engineering applications. However, the major limitations of this kind of hydrogels are low gel stability and weak mechanical properties under physiological conditions. Here, thermosensitive hydroxypropyl chitin (HPCH) was synthesized homogenously and subsequently functionalized with photocrosslinkable methacrylate groups via glycidyl methacrylate to generate glycidyl methacrylate-modified HPCH (GM-HPCH). The obtained new GM-HPCH polymers exhibited similar reversible thermosensitive sol-gel transition behaviors at a low concentration (2 wt% in PBS). The physical thermogelation GM-HPCH hydrogels were able to be photocrosslinked by UV irradiation under physiological conditions to form enhanced stable and mechanically strong hydrogels. The mechanical property, swelling and degradation behavior of the hydrogels could be tuned by controlling the degree of substitution of methacrylate groups and UV exposure time. Cytotoxicity test displayed that the photocrosslinked thermogels were non-cytotoxic. The photocrosslinkable GM-HPCH thermogels hold great potential for biomedical applications.

Keywords: Chitin (PubChem CID: 6857375); Glycidyl methacrylate (PubChem CID: 7837); Hydroxypropyl chitin; Injectable hydrogel; Irgacure 2959 (PubChem CID: 86266); Lysozyme (PubChem CID: 16129749); Methacrylation; Photocrosslinking; Propylene oxide (PubChem CID: 146261); Sodium hydroxide (PubChem CID:14798); Thermosensitive; Urea (PubChem CID: 1176).