Pathogen detection in the CRISPR-Cas era

Dipali G Sashital

doi:10.1186/s13073-018-0543-4

Pathogen detection in the CRISPR-Cas era

Genome Med. 2018 Apr 24;10(1):32. doi: 10.1186/s13073-018-0543-4.

Author

Dipali G Sashital¹

Affiliation

¹ Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, IA, 50011, USA. sashital@iastate.edu.

Abstract

CRISPR-Cas systems have provided revolutionary tools for genome editing. The discovery of Cas proteins with alternative activities has now enabled sensitive and robust tools for detecting nucleic acids. Recent reports harnessing these new CRISPR-Cas technologies display their potential for providing low-cost and practical diagnostic tools for pathogen and disease detection.

Keywords: Bacteria; Biotechnology; CRISPR–Cas; Diagnostics; Genotyping; Pathogen detection; Virus.

Publication types

Review

MeSH terms

Bacteria / isolation & purification*
CRISPR-Associated Proteins / metabolism*
CRISPR-Cas Systems / genetics*
Nucleic Acids / analysis
Viruses / isolation & purification*

Substances

CRISPR-Associated Proteins
Nucleic Acids

Grants and funding

R01 GM115874/GM/NIGMS NIH HHS/United States