Genome misclassification of Klebsiella variicola and Klebsiella quasipneumoniae isolated from plants, animals and humans

Salud Publica Mex. 2018 Jan-Feb;60(1):56-62. doi: 10.21149/8149.

Abstract

Objective: Due to the fact that K. variicola, K. quasipneumoniae and K. pneumoniae are closely related bacterial species, misclassification can occur due to mistakes either in normal biochemical tests or during submission to public databases. The objective of this work was to identify K. variicola and K. quasipneumoniae genomes misclassified in GenBank database.

Materials and methods: Both rpoB phylogenies and average nucleotide identity (ANI) were used to identify a significant number of misclassified Klebsiella spp. genomes.

Results: Here we report an update of K. variicola and K. Quasipneumoniae genomes correctly classified and a list of isolated genomes obtained from humans, plants, animals and insects, described originally as K. pneumoniae or K. variicola, but known now to be misclassified.

Conclusions: This work contributes to recognize the extensive presence of K. variicola and K. quasipneumoniae isolates in diverse sites and samples.

Keywords: Enterobacteriaceae infections; Gram-negative bacterial infections; bacterial infections; nitrogen fixation.

MeSH terms

  • Animals
  • Bacterial Typing Techniques*
  • DNA, Bacterial
  • Genome, Bacterial*
  • Humans
  • Insecta / microbiology*
  • Klebsiella / classification*
  • Klebsiella / genetics
  • Klebsiella / isolation & purification
  • Klebsiella Infections / microbiology*
  • Klebsiella Infections / veterinary
  • Phylogeny
  • Plants / microbiology*
  • Sequence Analysis, DNA
  • Species Specificity
  • Ursidae / microbiology*

Substances

  • DNA, Bacterial