A robust electrochemical immunosensor based on hydroxyl pillar[5]arene@AuNPs@g-C3N4 hybrid nanomaterial for ultrasensitive detection of prostate specific antigen

Xu Zhou; Long Yang; Xiaoping Tan; Genfu Zhao; Xiaoguang Xie; Guanben Du

doi:10.1016/j.bios.2018.04.036

A robust electrochemical immunosensor based on hydroxyl pillar[5]arene@AuNPs@g-C₃N₄ hybrid nanomaterial for ultrasensitive detection of prostate specific antigen

Biosens Bioelectron. 2018 Jul 30:112:31-39. doi: 10.1016/j.bios.2018.04.036. Epub 2018 Apr 18.

Authors

Xu Zhou¹, Long Yang², Xiaoping Tan³, Genfu Zhao⁴, Xiaoguang Xie⁵, Guanben Du⁶

Affiliations

¹ Key Lab for Forest Resources Conservation and Utilization in the Southwest Mountains, Ministry of Education, Yunnan Province Key Lab of Wood Adhesives and Glued Products, School of Materials Science and Engineering, Southwest Forestry University, Kunming 650224, China; School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
² Key Lab for Forest Resources Conservation and Utilization in the Southwest Mountains, Ministry of Education, Yunnan Province Key Lab of Wood Adhesives and Glued Products, School of Materials Science and Engineering, Southwest Forestry University, Kunming 650224, China; Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China. Electronic address: long133109070@126.com.
³ Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China.
⁴ Key Lab for Forest Resources Conservation and Utilization in the Southwest Mountains, Ministry of Education, Yunnan Province Key Lab of Wood Adhesives and Glued Products, School of Materials Science and Engineering, Southwest Forestry University, Kunming 650224, China.
⁵ School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
⁶ Key Lab for Forest Resources Conservation and Utilization in the Southwest Mountains, Ministry of Education, Yunnan Province Key Lab of Wood Adhesives and Glued Products, School of Materials Science and Engineering, Southwest Forestry University, Kunming 650224, China. Electronic address: guanben@swfu.edu.cn.

PMID: 29689502
DOI: 10.1016/j.bios.2018.04.036

Abstract

Prostate specific antigen (PSA) is the most significant biomarker for the screening of prostate cancer in human serum. However, most methods for the detection of PSA often require major laboratories, precisely analytical instruments and complicated operations. Currently, the design and development of satisfying electrochemical biosensors based on biomimetic materials (e.g. synthetic receptors) and nanotechnology is highly desired. Thus, we focused on the combination of molecular recognition and versatile nanomaterials in electrochemical devices for advancing their analytical performance and robustness. Herein, by using the present prepared multifunctional hydroxyl pillar[5]arene@gold nanoparticles@graphitic carbon nitride (HP5@AuNPs@g-C₃N₄) hybrid nanomaterial as robust biomimetic element, a high-performance electrochemical immunosensor for detection of PSA was constructed. The as-prepared immunosensor, with typically competitive advantages of low cost, simple preparation and fast detection, exhibited remarkable robustness, ultra-sensitivity, excellent selectivity and reproducibility. The limit of detection (LOD) and linear range were 0.12 pg mL^-1 (S/N = 3) and 0.0005-10.00 ng mL^-1, respectively. The satisfying results provide a promising approach for clinical detection of PSA in human serum.

Keywords: Graphitic carbon nitride; Hybrid nanomaterial; Immunosensor; Pillar[5]arene; Prostate specific antigen.

MeSH terms

Antibodies, Immobilized / chemistry
Biosensing Techniques*
Electrochemical Techniques*
Gold / chemistry
Humans
Immunoassay / methods
Limit of Detection
Male
Metal Nanoparticles / chemistry
Prostate-Specific Antigen / chemistry
Prostate-Specific Antigen / isolation & purification*
Prostatic Neoplasms / diagnosis*

Substances

Antibodies, Immobilized
Gold
Prostate-Specific Antigen