Capturing platinum in cisplatin: kinetic reactions with recombinant human apo-metallothionein 1a

Metallomics. 2018 May 23;10(5):713-721. doi: 10.1039/c8mt00029h.

Abstract

cis-Diamminedichloroplatinum(ii) (cisplatin), a powerful chemotherapeutic, can incur chemoresistance in cancers, reducing therapeutic success. Metallothioneins (MTs) are suspected of metallodrug interference via ligand removal and metal sequestration. The mechanistic details and reactions rates kobs for the systematic deconstruction of cisplatin by apo-human MT are reported and analysed from mass spectral data.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / pharmacology
  • Apoproteins / chemistry
  • Apoproteins / metabolism
  • Cisplatin / chemistry*
  • Cisplatin / pharmacology
  • Humans
  • Kinetics
  • Metallothionein / chemistry
  • Metallothionein / metabolism*
  • Metals / metabolism
  • Neoplasms / drug therapy
  • Neoplasms / metabolism
  • Neoplasms / pathology
  • Platinum / chemistry*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism*

Substances

  • Antineoplastic Agents
  • Apoproteins
  • MT1A protein, human
  • Metals
  • Recombinant Proteins
  • Platinum
  • Metallothionein
  • Cisplatin