MS-based conformation analysis of recombinant proteins in design, optimization and development of biopharmaceuticals

Abstract

Mass spectrometry (MS)-based methods for analyzing protein higher order structures have gained increasing application in the field of biopharmaceutical development. The predominant methods used in this area include native MS, hydrogen deuterium exchange-MS, covalent labeling, cross-linking and limited proteolysis. These MS-based methods will be briefly described in this article, followed by a discussion on how these methods contribute at different stages of discovery and development of protein therapeutics.

Keywords: Antibody; Biopharmaceutical; Higher order structure; Hydrogen/deuterium exchange; Native mass spectrometry; Oxidative footprinting.