Interferometric temporal focusing microscopy using three-photon excitation fluorescence

Keisuke Toda; Keisuke Isobe; Kana Namiki; Hiroyuki Kawano; Atsushi Miyawaki; Katsumi Midorikawa

doi:10.1364/BOE.9.001510

Interferometric temporal focusing microscopy using three-photon excitation fluorescence

Biomed Opt Express. 2018 Mar 6;9(4):1510-1519. doi: 10.1364/BOE.9.001510. eCollection 2018 Apr 1.

Authors

Keisuke Toda^{1

2}, Keisuke Isobe^{1

3}, Kana Namiki⁴, Hiroyuki Kawano⁴, Atsushi Miyawaki^{1

4}, Katsumi Midorikawa^{1

2}

Affiliations

¹ RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
² Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura, Saitama 338-8570, Japan.
³ JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan.
⁴ Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Abstract

Super-resolution microscopy has become a powerful tool for biological research. However, its spatial resolution and imaging depth are limited, largely due to background light. Interferometric temporal focusing (ITF) microscopy, which combines structured illumination microscopy and three-photon excitation fluorescence microscopy, can overcome these limitations. Here, we demonstrate ITF microscopy using three-photon excitation fluorescence, which has a spatial resolution of 106 nm at an imaging depth of 100 µm with an excitation wavelength of 1060 nm.

Keywords: (100.6640) Superresolution; (180.4315) Nonlinear microscopy; (180.6900) Three-dimensional microscopy.