Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregates in vitro

Madhuri Chakravarthy; Hadi AlShamaileh; He Huang; Rudi K Tannenberg; Suxiang Chen; Simon Worrall; Peter R Dodd; Rakesh N Veedu

doi:10.1039/c8cc02256a

Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregates in vitro

Chem Commun (Camb). 2018 May 1;54(36):4593-4596. doi: 10.1039/c8cc02256a.

Authors

Madhuri Chakravarthy¹, Hadi AlShamaileh, He Huang, Rudi K Tannenberg, Suxiang Chen, Simon Worrall, Peter R Dodd, Rakesh N Veedu

Affiliation

¹ Centre for Comparative Genomics, Murdoch University, Perth, 6150, Australia. R.Veedu@murdoch.edu.au.

PMID: 29670956
DOI: 10.1039/c8cc02256a

Abstract

We have developed a novel functional nucleic acid aptamer to amyloid-β peptide 1-40 (Aβ1-40) and investigated its potential to detect Aβ peptide fragments in neuropathologically confirmed Alzheimer brain hippocampus tissues samples. Our results demonstrate that the aptamer candidate RNV95 could detect tetrameric/pentameric low-molecular-weight Aβ aggregates in autopsy hippocampal tissue from two neuropathologically confirmed Alzheimer disease cases. Although these are preliminary observations, detailed investigations are under way. This is the first demonstration of aptamer-Aβ binding in Alzheimer brain tissues.

MeSH terms

Alzheimer Disease / diagnostic imaging*
Alzheimer Disease / pathology
Amyloid / chemistry
Amyloid / metabolism*
Amyloid beta-Peptides / chemistry
Amyloid beta-Peptides / metabolism*
Aptamers, Nucleotide / chemistry
Aptamers, Nucleotide / metabolism*
Hippocampus / pathology*
Humans
Peptide Fragments / chemistry
Peptide Fragments / metabolism*
Plaque, Amyloid / diagnostic imaging
Plaque, Amyloid / pathology
Protein Aggregation, Pathological / metabolism
Protein Binding
Protein Multimerization

Substances

Amyloid
Amyloid beta-Peptides
Aptamers, Nucleotide
Peptide Fragments
amyloid beta-protein (1-40)