Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia

Dina M Metwally; Latifa E Qassim; Isra M Al-Turaiki; Rafa S Almeer; Manal F El-Khadragy

doi:10.1371/journal.pone.0195016

Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia

PLoS One. 2018 Apr 18;13(4):e0195016. doi: 10.1371/journal.pone.0195016. eCollection 2018.

Authors

Dina M Metwally^{1

2}, Latifa E Qassim¹, Isra M Al-Turaiki³, Rafa S Almeer¹, Manal F El-Khadragy^{1

4}

Affiliations

¹ Zoology Department, Faculty of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
² Parasitology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
³ Information Technology Department, College of Computer and Information Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
⁴ Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.

Abstract

The diversity and importance of Echinococcus species in domesticated animals in Saudi Arabia are poorly understood. In this study, 108 singular (hydatid) cysts were collected from goats (n = 25), sheep (n = 56) and camels (n = 27). DNA was extracted from the protoscoleces of individual fertile cysts and used for polymerase chain reaction (PCR) amplification of mitochondrial subunit 1 of the cytochrome c oxidase 1 (cox1) gene. Amplicon sequencing results revealed the presence of Echinococcus granulosus sensustricto (s.s.) (genotypes G1-G3) in 16 of the17 sheep cysts and 2 of the 27 camel cysts.of these samples, 18 (2 camel and 16 sheep) were divided into genotypes G1, G2, and G3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Diseases / epidemiology*
Animal Diseases / parasitology*
Animals
Base Sequence
Camelus
Echinococcosis / veterinary*
Echinococcus granulosus / classification*
Echinococcus granulosus / genetics*
Echinococcus granulosus / isolation & purification
Electron Transport Complex IV / chemistry
Electron Transport Complex IV / genetics*
Genotype
Livestock / parasitology*
Phylogeny
Polymerase Chain Reaction
Saudi Arabia / epidemiology
Sequence Analysis, DNA
Sheep

Substances

Electron Transport Complex IV

Grants and funding

This research project was supported by a grant from the Deanship of Scientific Research at King Saud University for funding this research through the Research Group project no. RG-1439-034. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.