[Inhibitory Effect of miR-125b Down-Regulation on Proliferation of Leukemia Cell K562]

Jie Liu; Chang-Qing Tong

doi:10.7534/j.issn.1009-2137.2018.02.005

[Inhibitory Effect of miR-125b Down-Regulation on Proliferation of Leukemia Cell K562]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Apr;26(2):336-340. doi: 10.7534/j.issn.1009-2137.2018.02.005.

[Article in Chinese]

Authors

Jie Liu¹, Chang-Qing Tong²

Affiliations

¹ Department of Hematology,The First Affiliated Hospital of Hebei North University, Zhangjiako 075000, Hebei Province, China.
² Department of Hematology,The First Affiliated Hospital of Hebei North University, Zhangjiako 075000, Hebei Province, China. E-mail:liujie_0313@163.com.

PMID: 29665895
DOI: 10.7534/j.issn.1009-2137.2018.02.005

Abstract

Objective: To explore inhibitory effect of miR-125b down-regulation on proliferation of leukemia cell K562.

Methods: miR-125b inhibitor and miR-125b NC were transfected into K562 cells by liposome Lipofectamine^TM2000, the cell viability was measured by MTT assay, cell cloning ability was detected by agarose cloning assay, cell cycle was measured by flow cytometry. The expression of BCL-2, BCL-2 homology antagonist/liller 1(BAK1), p53 and p53 up-regulated modulator of apoptosis (Puma) was measured by Western blot.

Results: Compared with miR-125b NC, the expression of miR-125b was down-regulated (P<0.01), the cell viability and cell cloning ability were reduced (P<0.01), the cell cycle was arrested in the G₁ phase (P<0.01), the expression of BCL-2 were down-regulated (P<0.01), the expression of BAK1, p53 and Puma were up-regulated in miR-125b inhibitor group (P<0.01).

Conclusion: Down-regulation of miR-125b can inhibit K562 cell proliferation via down-regulating the expressions of BCL-2 and up-regulating the expression of BAK1, p53 and Puma.

MeSH terms

Apoptosis
Cell Line, Tumor
Cell Proliferation*
Down-Regulation
Humans
Leukemia
MicroRNAs

Substances

MicroRNAs