Isolation of Oropouche Virus from Febrile Patient, Ecuador

Emerg Infect Dis. 2018 May;24(5):935-937. doi: 10.3201/eid2405.171569.

Abstract

We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

Keywords: Arbovirus; Bunyaviridae; Ecuador; Oropouche; Orthobunyaviridae; South America; bunyavirus; communicable disease; high-throughput nucleotide sequencing; metagenomic; orthobunyavirus; virus; viruses.

Publication types

  • Case Reports
  • Letter

MeSH terms

  • Adult
  • Animals
  • Bunyaviridae Infections / epidemiology
  • Bunyaviridae Infections / virology*
  • Chlorocebus aethiops
  • Ecuador / epidemiology
  • Humans
  • Male
  • Orthobunyavirus / genetics
  • Orthobunyavirus / isolation & purification*
  • Phylogeny
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Vero Cells

Supplementary concepts

  • Oropouche orthobunyavirus