Multi-hierarchical tissue-engineering ECM-like scaffolds based on cellulose acetate with collagen and chitosan fillers

Abstract

A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared. The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler. The fibrous component was prepared by electrospinning, the sponge - freeze-drying technique. The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc. on the fiber-sponge architecture and properties was examined. It was shown that scaffolds with different types of microstructure can be obtained employing this technique. The impregnation of chitosan or collagen filler in fiber matrix also significantly improves mechanical properties up to 40 MPa for strength and 600 MPa for Young's modulus.

Keywords: Acetic acid (PubChem CID:176); Cellulose acetate; Chitosan; Chitosan (PubChem CID:21896651); Collagen; Collagen (PubChem CID:78759337); Ethyl acetate (PubChem CID:8857); Fiber-sponge scaffold; Mechanical properties; Spatial architecture.