A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode

Yue Wang; Fengge Zhai; Yasushi Hasebe; Hongmin Jia; Zhiqiang Zhang

doi:10.1016/j.bioelechem.2018.04.003

A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode

Bioelectrochemistry. 2018 Aug:122:174-182. doi: 10.1016/j.bioelechem.2018.04.003. Epub 2018 Apr 9.

Authors

Yue Wang¹, Fengge Zhai², Yasushi Hasebe³, Hongmin Jia², Zhiqiang Zhang⁴

Affiliations

¹ School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, Liaoning 114051, China. Electronic address: wangyue@ustl.edu.cn.
² School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, Liaoning 114051, China.
³ Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan.
⁴ School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, Liaoning 114051, China. Electronic address: zhangzhiqiang@ustl.edu.cn.

PMID: 29656242
DOI: 10.1016/j.bioelechem.2018.04.003

Abstract

The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5×10^-8M to 5×10^-5M, a lower detection limit of 3×10^-8M, a current maximum (I_max) of 65.8μA and an apparent Michaelis constant (K_m^app) of 169.9μM.

Keywords: Covalent bonding; Glassy carbon electrode; Graphene oxide; Phenol derivatives; Tyrosinase.

MeSH terms

Agaricales / enzymology*
Biosensing Techniques / instrumentation
Biosensing Techniques / methods*
Catechols / analysis
Electrodes
Enzymes, Immobilized / chemistry
Graphite / chemistry*
Limit of Detection
Monophenol Monooxygenase / chemistry*
Oxides / chemistry
Phenols / analysis*

Substances

Catechols
Enzymes, Immobilized
Oxides
Phenols
Graphite
Monophenol Monooxygenase
catechol