Transcriptomic and Ectoine Analysis of Halotolerant Nocardiopsis gilva YIM 90087T Under Salt Stress

Jian Han; Quan-Xiu Gao; Yong-Guang Zhang; Li Li; Osama A A Mohamad; Manik Prabhu Narsing Rao; Min Xiao; Wael N Hozzein; Dalal H M Alkhalifah; Yong Tao; Wen-Jun Li

doi:10.3389/fmicb.2018.00618

Transcriptomic and Ectoine Analysis of Halotolerant Nocardiopsis gilva YIM 90087^T Under Salt Stress

Front Microbiol. 2018 Mar 29:9:618. doi: 10.3389/fmicb.2018.00618. eCollection 2018.

Authors

Jian Han^{1

2

3}, Quan-Xiu Gao², Yong-Guang Zhang¹, Li Li¹, Osama A A Mohamad^{1

4}, Manik Prabhu Narsing Rao⁵, Min Xiao⁵, Wael N Hozzein^{6

7}, Dalal H M Alkhalifah⁸, Yong Tao², Wen-Jun Li^{1

5}

Affiliations

¹ Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, China.
² Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
³ University of Chinese Academy of Sciences, Beijing, China.
⁴ Institute for Post Graduate Environmental Studies, Environmental Science Department, Arish University, North Sinai, Egypt.
⁵ State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
⁶ Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt.
⁷ Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
⁸ Biology Department, Faculty of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

Abstract

The genus Nocardiopsis is an unique actinobacterial group that widely distributed in hypersaline environments. In this study, we investigated the growth conditions, transcriptome analysis, production and accumulation of ectoine by Nocardiopsis gilva YIM 90087^T under salt stress. The colony color of N. gilva YIM 90087^T changed from yellow to white under salt stress conditions. Accumulation of ectoine and hydroxyectoine in cells was an efficient way to regulate osmotic pressure. The ectoine synthesis was studied by transferring the related genes (ectA, ectB, and ectC) to Escherichia coli. Transcriptomic analysis showed that the pathways of ABC transporters (ko02010) and glycine, serine, and threonine metabolism (ko00260) played a vital role under salt stress environment. The ectABC from N. gilva YIM 90087^T was activated under the salt stress. Addition of exogenous ectoine and hydroxyectoine were helpful to protect N. gilva YIM 90087^T from salt stress.

Keywords: Nocardiopsis gilva; ectoine; halotolerant; hydroxyectoine; transcriptomic analysis; whole-cell catalysis.