Preparation, characterization and in vitro release of β-galactosidase loaded polyelectrolyte nanoparticles

Hongcai Zhang; Huaning Yu; Jun Mei; Yifeng Zhang; Zilong Deng

doi:10.1016/j.ijbiomac.2018.04.030

Preparation, characterization and in vitro release of β-galactosidase loaded polyelectrolyte nanoparticles

Int J Biol Macromol. 2018 Aug:115:1-9. doi: 10.1016/j.ijbiomac.2018.04.030. Epub 2018 Apr 10.

Authors

Hongcai Zhang¹, Huaning Yu², Jun Mei³, Yifeng Zhang⁴, Zilong Deng⁵

Affiliations

¹ State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Synergetic Innovation Center of Food Safety and Nutrition, Shanghai 200436, China; College of Food Science and Technology, Shanghai Ocean University, No 999 Huchenghuan Road, Lingang New District, Shanghai 201306, China. Electronic address: hczhang@shou.edu.cn.
² State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Synergetic Innovation Center of Food Safety and Nutrition, Shanghai 200436, China.
³ College of Food Science and Technology, Shanghai Ocean University, No 999 Huchenghuan Road, Lingang New District, Shanghai 201306, China.
⁴ Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China.
⁵ Department of Food Science and Technology, Oregon State University, Corvallis 97331-6602, USA. Electronic address: davidzilongdeng@hotmail.com.

PMID: 29649531
DOI: 10.1016/j.ijbiomac.2018.04.030

Abstract

Improving encapsulation efficacy (EE) and bioavailability of β-galactosidase (β-gal) is always a challenge due to its fragility. In this work, β-gal loaded β-chitosan (CS) nanoparticles (NPs) were successfully prepared based on ionic gelation technique and electrostatic attraction for improving its EE and in vitro releasing capacity. The particle size of β-gal loaded low and high molecular weight (LMW and HMW) β-CS NPs reached 584.37 and 652.46nm, with Zeta-potential (ZP) of 26.37 and 16.46mV under the optimal conditions, respectively. In vitro release study conducted at pH4.5 and 7.4 showed that β-gal loaded LMW and HMW β-CS NPs with EE of 68.32 and 58.64% sustained the release of the β-gal over 12h. The β-gal incorporated into β-CS NPs was confirmed with the results of physicochemical and structural properties of β-gal loaded β-CS NPs, and prepared NPs had hardly any cytotoxicity in the range of 0.1-1.0mg/mL. The results indicated that β-gal loaded β-CS NPs could serve as non-toxic delivery carriers for the treatment of lactose intolerance.

Keywords: Encapsulation efficacy; In vitro release; Nanoparticles; β-chitosan; β-galactosidase.

MeSH terms

Cell Line
Cell Survival / drug effects
Drug Carriers / chemistry*
Drug Carriers / toxicity
Drug Liberation*
Endothelial Cells / cytology
Endothelial Cells / drug effects
Molecular Weight
Nanoparticles / chemistry*
Particle Size
Polyelectrolytes / chemistry*
Polyelectrolytes / toxicity
beta-Galactosidase / chemistry*

Substances

Drug Carriers
Polyelectrolytes
beta-Galactosidase