Occurrence of Salmonella genomic island 1 (SGI1) in two African Proteus mirabilis strains isolated from diseased chicken flocks

Ahmed M Soliman; Alaaeldin Mohamed Saad; Ashraf M Ahmed; Ahmed Al-Baqir; Ashraf Hussein; Toshi Shimamoto; Hirofumi Nariya; Tadashi Shimamoto

doi:10.1016/j.meegid.2018.04.008

Occurrence of Salmonella genomic island 1 (SGI1) in two African Proteus mirabilis strains isolated from diseased chicken flocks

Infect Genet Evol. 2018 Aug:62:8-10. doi: 10.1016/j.meegid.2018.04.008. Epub 2018 Apr 8.

Authors

Ahmed M Soliman¹, Alaaeldin Mohamed Saad², Ashraf M Ahmed³, Ahmed Al-Baqir⁴, Ashraf Hussein⁴, Toshi Shimamoto⁵, Hirofumi Nariya⁵, Tadashi Shimamoto⁶

Affiliations

¹ Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan; Department of Microbiology and Immunology, Faculty of Pharmacy, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
² Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8539, Japan.
³ Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
⁴ Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
⁵ Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
⁶ Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan. Electronic address: tadashis@hiroshima-u.ac.jp.

PMID: 29641984
DOI: 10.1016/j.meegid.2018.04.008

Abstract

Two P. mirabilis strains, PmSHR21 and PmSHR38, were collected from chicken flocks in Sharkia Governorate, Egypt in 2016. The two strains showed multidrug-resistance (MDR) phenotypes and were detected to harbour I) floR and sul1 genes conferring resistance to florfenicol and chloramphenicol, and sulfonamides, respectively, II) a ~1.9 kbp class 1 integron containing aadA2-lnuF genes conferring resistance to spectinomycin and streptomycin, and lincosamides, respectively. Interestingly, the two strains were detected to contain SGI1 variant, SGI1-W and inserted between the 3' end of the chromosomal trmE gene and the hipB/hipA toxin/antitoxin homologue. Fingerprinting by ERIC-PCR of the two poultry strains identified in this study and the two human SGI1-carrying P. mirabilis strains described recently in our study showed identical ERIC-pattern between SGI1-W-carrying poultry and human strains, suggesting that they might be clonally related. The detection of SGI1 and its variants in P. mirabilis isolated from humans and chicken flocks in Egypt clarify the geographical and biological spreading through an inter-transmission pathway. To the best of our knowledge, this is the first study detecting SGI1-positive P. mirabilis isolated from chicken flocks in Africa.

Keywords: Africa; Chicken; Egypt; Poultry; Proteus mirabilis; SGI1-W; hipBA; trmE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens*
Egypt / epidemiology
Genomic Islands
Poultry Diseases / epidemiology
Poultry Diseases / microbiology*
Proteus Infections / epidemiology
Proteus Infections / veterinary*
Proteus mirabilis / genetics*
Salmonella / genetics*