Enterobacter aerogenes metabolites enhance Microcystis aeruginosa biomass recovery for sustainable bioflocculant and biohydrogen production

Liang Xu; Mo Zhou; Hanyu Ju; Zhenxing Zhang; Jiquan Zhang; Caiyun Sun

doi:10.1016/j.scitotenv.2018.03.327

Enterobacter aerogenes metabolites enhance Microcystis aeruginosa biomass recovery for sustainable bioflocculant and biohydrogen production

Sci Total Environ. 2018 Sep 1:634:488-496. doi: 10.1016/j.scitotenv.2018.03.327. Epub 2018 Apr 7.

Authors

Liang Xu¹, Mo Zhou², Hanyu Ju², Zhenxing Zhang³, Jiquan Zhang⁴, Caiyun Sun⁵

Affiliations

¹ School of Environment, Northeast Normal University, Changchun 130117, China; Jilin Institute of Chemical Technology, Jilin, 132022, China; Key Laboratory for Vegetation Ecology, Ministry of Education, NO. 2555 Jingyue Street, Changchun 130117, China; State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, Northeast Normal University, Changchun, 130024, China.
² School of Environment, Northeast Normal University, Changchun 130117, China; Key Laboratory for Vegetation Ecology, Ministry of Education, NO. 2555 Jingyue Street, Changchun 130117, China; State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, Northeast Normal University, Changchun, 130024, China.
³ Institute of Grassland Science, Northeast Normal University, Key Laboratory of Vegetation Ecology, China; Key Laboratory for Vegetation Ecology, Ministry of Education, NO. 2555 Jingyue Street, Changchun 130117, China.
⁴ School of Environment, Northeast Normal University, Changchun 130117, China; Key Laboratory for Vegetation Ecology, Ministry of Education, NO. 2555 Jingyue Street, Changchun 130117, China; State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, Northeast Normal University, Changchun, 130024, China. Electronic address: zhangjq022@nenu.edu.cn.
⁵ School of Environment, Northeast Normal University, Changchun 130117, China; Jilin Institute of Chemical Technology, Jilin, 132022, China; Key Laboratory for Vegetation Ecology, Ministry of Education, NO. 2555 Jingyue Street, Changchun 130117, China; State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, Northeast Normal University, Changchun, 130024, China. Electronic address: 437621315@qq.com.

PMID: 29635192
DOI: 10.1016/j.scitotenv.2018.03.327

Abstract

We report a recycling bioresource involving harvesting of Microcystis aeruginosa using the bioflocculant (MBF-32) produced by Enterobacter aerogenes followed by the recovery of the harvested M. aeruginosa as the main substrate for the sustainable production of MBF-32 and biohydrogen. The experimental results indicate that the efficiency of bioflocculation exceeded 90% under optimal conditions. The harvested M. aeruginosa was further recycled as the main substrate for the supply of necessary elements. The highest yield (3.6±0.1g/L) of MBF-32 could be obtained from 20g/L of wet biomass of M. aeruginosa with an additional 20g/L of glucose as the extra carbon source. The highest yield of biohydrogen was 35mL of H₂/g (dw) algal biomass, obtained from 20g/L of wet biomass of M. aeruginosa with an additional 10g/L of glycerol. Transcriptome analyses indicated that MBF-32 was mainly composed of polysaccharide and tyrosine/tryptophan proteins. Furthermore, NADH synthase and polysaccharide export-related genes were found to be up-regulated.

Keywords: Bioflocculation; Biohydrogen; Bioresource recycling; E. aerogenes; Metabolic pathways; Transcriptome.

MeSH terms

Biofuels*
Biomass
Enterobacter aerogenes / physiology*
Fermentation / physiology
Hydrogen / metabolism*
Microcystis / physiology*

Substances

Biofuels
Hydrogen