Monascus pigments (Mps) have been used as food colorants for several centuries in Asian countries. MptriA is a putative acetyltransferase gene involved in the MPs biosynthesis. To analyze the function of MptriA, an MptriA disruption strain (Δ MptriA) and a complementation strain (Δ MptriA:: MptriA) were successfully obtained In addition to the loss of color, the disruption of MptriA had little effect on the phenotypes during growth on four different media. The Δ MptriA strain showed decreased pigment and citrinin production during the liquid-fermentation process. Transcriptional analysis showed that the expression of several genes involved in the synthesis of pigments and citrinin was down-regulated in Δ MptriA. These results demonstrated that the role of MptriA was to transfer an acyl group to the pyranoquinone structure of the polyketide chromophore during Monascus pigment biosynthesis and to influence the citrinin biosynthesis pathway. This study contributes to the exploration of pigment biosynthesis in M. purpureus.
Keywords: M. purpureus; MptriA gene; complementation; disruption; pigments.