Abstract
Two new labdane diterpenoids, Leojaponin E (1) and F (2), together with three known compounds were isolated from the dried herb of Leonurus japonicus Houtt., Lamiaceae. Their structures were determined based on extensive spectroscopic analyses. The absolute configurations of 1 and 2 were elucidated on the basis of experimental and calculated electronic circular dichroism spectra. In addition, compounds 1 and 2 exerted inhibition of LPS-induced PGE2 production in a dose-dependent manner at concentrations ranging from 5 to 20 μM.
Keywords:
ECD; anti-inflammatory activity; labdane diterpenoids.
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Circular Dichroism
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Dinoprostone / biosynthesis
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Diterpenes / chemistry*
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Diterpenes / pharmacology*
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Dose-Response Relationship, Drug
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Drug Evaluation, Preclinical
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Leonurus / chemistry*
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Lipopolysaccharides / pharmacology
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Magnetic Resonance Spectroscopy
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Mice
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Molecular Structure
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Plant Components, Aerial / chemistry
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RAW 264.7 Cells
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Diterpenes
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Lipopolysaccharides
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Dinoprostone