Derivation and characterization of the NIH registry human stem cell line NYSCF100 line under defined feeder-free conditions

Ana Sevilla; Eliana Forero; Matthew Zimmer; Hector Martinez; Katie Reggio; Daniel Paull; Dieter Egli; Scott Noggle

doi:10.1016/j.scr.2018.03.017

Derivation and characterization of the NIH registry human stem cell line NYSCF100 line under defined feeder-free conditions

Stem Cell Res. 2018 May:29:99-102. doi: 10.1016/j.scr.2018.03.017. Epub 2018 Mar 31.

Authors

Ana Sevilla¹, Eliana Forero², Matthew Zimmer², Hector Martinez², Katie Reggio², Daniel Paull², Dieter Egli², Scott Noggle²

Affiliations

¹ The New York Stem Cell Foundation Research Institute, New York, NY 10019, United States. Electronic address: asevilla@nyscf.org.
² The New York Stem Cell Foundation Research Institute, New York, NY 10019, United States.

PMID: 29631040
DOI: 10.1016/j.scr.2018.03.017

Abstract

The human embryonic stem cell line NYSCFe001-A was derived from a day 6 blastocyst in feeder-free and antibiotic free conditions. The blastocyst was voluntarily donated for research as surplus after in vitro fertilization treatment following informed consent. The NYSCFe001-A line, registered as NYSCF100 on the NIH registry, presents normal karyotype, is mycoplasma free, expresses all the pluripotency markers and has the potential to differentiate into all three germ layers in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Human Embryonic Stem Cells / metabolism*
Humans
National Institutes of Health (U.S.)
Registries
United States