Abstract
We report a strategy to introduce a reactive electrophile into proteins through the conversion of a chemically inert group into a bioreactive group in response to an inducer molecule. This strategy was demonstrated by oxidation-induced and proximity-enhanced protein-protein crosslinking in the presence of a large excess of free nucleophile.
MeSH terms
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Catalysis
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Cross-Linking Reagents / chemical synthesis
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Cross-Linking Reagents / chemistry*
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Dansyl Compounds / chemistry
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Escherichia coli
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Ethylenediamines / chemistry
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Fluorescent Dyes / chemistry
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Glutathione Transferase / chemistry
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Glutathione Transferase / genetics
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / genetics
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Hydrogen Peroxide / chemistry
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Lysine / analogs & derivatives*
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Lysine / chemical synthesis
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Lysine / chemistry*
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Lysine / genetics*
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Methanosarcina barkeri
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Molecular Probes / chemistry*
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Molecular Probes / genetics
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Mutation
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Myoglobin / chemistry
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Myoglobin / genetics
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Oxidation-Reduction
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Proteins / chemistry*
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Proteins / genetics
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Sulfoxides / chemistry
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Tungsten Compounds / chemistry
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Vinyl Compounds / chemical synthesis
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Vinyl Compounds / chemistry*
Substances
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Cross-Linking Reagents
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Dansyl Compounds
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Ethylenediamines
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Fluorescent Dyes
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Molecular Probes
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Myoglobin
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Proteins
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Sulfoxides
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Tungsten Compounds
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Vinyl Compounds
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Green Fluorescent Proteins
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dansylethylenediamine
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Hydrogen Peroxide
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Glutathione Transferase
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Lysine