Generation of WAe001-A-15, a human embryonic stem cell line with miR-122 doxycycline-inducible expression

Stem Cell Res. 2018 May:29:84-87. doi: 10.1016/j.scr.2018.03.007. Epub 2018 Mar 23.

Abstract

MiR-122 is the most abundant miRNA in the human liver accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 is a valuable therapeutic target for liver diseases, including viral hepatitis, fibrosis, steatosis, and hepatocarcinoma. Here, we constructed a miR-122 doxycycline-inducible expression human embryonic stem cell line WAe001-A-15 using the piggyBac transposon system. The cell line retained its pluripotency, in vitro differentiation potential, normal morphology, and karyotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Cell Line
  • DNA Transposable Elements
  • Doxycycline / pharmacology*
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / drug effects
  • Embryonic Stem Cells / metabolism*
  • Humans
  • MicroRNAs / biosynthesis*
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / drug effects
  • Pluripotent Stem Cells / metabolism*

Substances

  • Anti-Bacterial Agents
  • DNA Transposable Elements
  • MIRN122 microRNA, human
  • MicroRNAs
  • Doxycycline