A single-step transconjugation system for gene deletion in Aggregatibacter actinomycetemcomitans

Nico S Stumpp; Sarah A Konze; Rita Gerardy-Schahn; Meike Stiesch; Falk F R Buettner

doi:10.1016/j.mimet.2018.04.002

A single-step transconjugation system for gene deletion in Aggregatibacter actinomycetemcomitans

J Microbiol Methods. 2018 May:148:74-77. doi: 10.1016/j.mimet.2018.04.002. Epub 2018 Apr 5.

Authors

Nico S Stumpp¹, Sarah A Konze², Rita Gerardy-Schahn², Meike Stiesch³, Falk F R Buettner⁴

Affiliations

¹ Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Germany.
² Institute of Clinical Biochemistry, Hannover Medical School, Germany.
³ Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Germany. Electronic address: stiesch.meike@mh-hannover.de.
⁴ Institute of Clinical Biochemistry, Hannover Medical School, Germany. Electronic address: buettner.falk@mh-hannover.de.

PMID: 29627600
DOI: 10.1016/j.mimet.2018.04.002

Abstract

Aggregatibacter (A.) actinomycetemcomitans is a periodontopathogenic bacterium causing aggressive periodontitis. Here we describe a single-step transconjugation system as novel and easily applicable protocol for site-specific genetic manipulation of A. actinomycetemcomitans. Deletion of PgaC, which is involved in the synthesis of biofilm matrix, led to a reduced biofilm formation.

Keywords: A. Actinomycetemcomitans; Biofilm; Gene deletion; PGA; Transconjugation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aggregatibacter actinomycetemcomitans / genetics*
Aggregatibacter actinomycetemcomitans / growth & development
Bacterial Proteins / genetics
Biofilms / growth & development
Gene Deletion*
Gene Knockout Techniques / methods*

Substances

Bacterial Proteins