Abstract
Two new α-pyrones, dothideopyrones E (1) and F (2), were isolated from a culture of the endolichenic fungus Dothideomycetes sp. EL003334. Their structures were elucidated by spectroscopic data analysis. Their absolute configurations were established by the modified Mosher's method. Compound 2 inhibited nitric oxide (NO) production with IC50 values of 15.0 ± 2.8 μM in lipopolysaccharide (LPS)-induced BV2 cells. Compound 2 diminished the protein expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Additionally, 2 decreased the mRNA expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology
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Biological Factors / chemistry*
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Biological Factors / pharmacology
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Cell Line
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Cyclooxygenase 2 / metabolism
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Fungi / chemistry*
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Interleukin-1beta / metabolism
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Interleukin-6 / metabolism
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Lipopolysaccharides / pharmacology
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Mice
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / metabolism
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Pyrones / chemistry*
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Biological Factors
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Interleukin-1beta
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Interleukin-6
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Lipopolysaccharides
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Pyrones
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Tumor Necrosis Factor-alpha
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Nitric Oxide
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2