Complete genome sequence of Bacillus thuringiensis L-7601, a wild strain with high production of melanin

Zhang-Lei Cao; Tong-Tong Tan; Kun Jiang; Si-Qi Mei; Xiao-Yue Hou; Jun Cai

doi:10.1016/j.jbiotec.2018.03.020

Complete genome sequence of Bacillus thuringiensis L-7601, a wild strain with high production of melanin

J Biotechnol. 2018 Jun 10:275:40-43. doi: 10.1016/j.jbiotec.2018.03.020. Epub 2018 Mar 31.

Authors

Zhang-Lei Cao¹, Tong-Tong Tan¹, Kun Jiang¹, Si-Qi Mei¹, Xiao-Yue Hou¹, Jun Cai²

Affiliations

¹ Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China.
² Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, China. Electronic address: caijun@nankai.edu.cn.

PMID: 29614251
DOI: 10.1016/j.jbiotec.2018.03.020

Abstract

Bacillus thuringiensis L-7601 (B. thuringiensis L-7601), belonging to Bacillus thuringiensis subsp. dendrolimus serotype H4a4b, is a wild-type strain which has the ability to produce melanin during the exponential phase of growth. The melanin produced is an excellent UV protective agent for the crystal insecticidal proteins. Here, we report the complete genome of B. thuringiensis L-7601 including one 5,790,408 bp chromosome and three plasmids. 6,519 CDSs and 150 RNA genes, including 106 tRNA genes, 39 rRNA genes and 5 ncRNA genes, were identified from the whole genome. In addition, our results indicated that homogentisic acid pathway is the melanogenic pathway in B. thuringiensis and accumulation of melanin is the consequence of hmgA frameshift mutant.

Keywords: Bacillus thuringiensis; Complete genome; Frameshift mutant; Melanin biosynthesis pathway; hmgA.

MeSH terms

Bacillus thuringiensis / genetics*
Bacillus thuringiensis / metabolism
Base Composition
Chromosomes, Bacterial
Genome Size
Melanins / biosynthesis*
Plasmids / genetics
Sequence Analysis, DNA / methods*

Substances

Melanins