A novel Triclosan Methacrylate-based composite reduces the virulence of Streptococcus mutans biofilm

Isaac Jordão de Souza Araújo; Andréia Bolzan de Paula; Roberta Caroline Bruschi Alonso; Jesus Roberto Taparelli; Lúcia Helena Innocentini Mei; Rafael Nóbrega Stipp; Regina Maria Puppin-Rontani

doi:10.1371/journal.pone.0195244

A novel Triclosan Methacrylate-based composite reduces the virulence of Streptococcus mutans biofilm

PLoS One. 2018 Apr 2;13(4):e0195244. doi: 10.1371/journal.pone.0195244. eCollection 2018.

Authors

Isaac Jordão de Souza Araújo¹, Andréia Bolzan de Paula¹, Roberta Caroline Bruschi Alonso^{2

3}, Jesus Roberto Taparelli⁴, Lúcia Helena Innocentini Mei⁴, Rafael Nóbrega Stipp⁵, Regina Maria Puppin-Rontani⁶

Affiliations

¹ Dental Materials Division, Operative Dentistry Department, Piracicaba Dental School, State University of Campinas. Piracicaba, São Paulo-Brazil.
² Department of Dentistry, School of Dentistry, Metropolitan University of Santos (UNIMES). Santos, São Paulo-Brazil.
³ Technological Research Center, School of Dentistry, University of Mogi das Cruzes (UMC). Mogi das Cruzes, São Paulo-Brazil.
⁴ Department of Materials Engineering and Bioprocess, Chemical Engineering School, State University of Campinas. Campinas, São Paulo-Brazil.
⁵ Department of Oral Diagnosis, Piracicaba Dental School, State University of Campinas. Piracicaba, São Paulo-Brazil.
⁶ Department of Pediatric Dentistry, Piracicaba Dental School, State University of Campinas. Piracicaba, São Paulo-Brazil.

Abstract

The use of antimicrobial monomers, linked to the polymer chain of resin composites, is an interesting approach to circumvent the effects of bacteria on the dental and material surfaces. In addition, it can likely reduce the incidence of recurrent caries lesions. The aim of this study was to evaluate the effects of a novel Triclosan Methacrylate (TM) monomer, which was developed and incorporated into an experimental resin composite, on Streptococcus mutans (S. mutans) biofilms, focusing on the analyses of vicR, gtfD, gtfC, covR, and gbpB gene expression, cell viability and biofilm characteristics. The contact time between TM-composite and S. mutans down-regulated the gbpB and covR and up-regulated the gtfC gene expression, reduced cell viability and significantly decreased parameters of the structure and characteristics of S. mutans biofilm virulence. The presence of Triclosan Methacrylate monomer causes harmful effects at molecular and cellular levels in S. mutans, implying a reduction in the virulence of those microorganisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / metabolism
Biofilms / drug effects*
Gene Expression Regulation, Bacterial / drug effects
Microscopy, Confocal
Polyethylene Glycols / pharmacology*
Polymethacrylic Acids / pharmacology*
Streptococcus mutans / drug effects*
Streptococcus mutans / growth & development
Streptococcus mutans / pathogenicity*
Streptococcus mutans / physiology
Virulence

Substances

Anti-Bacterial Agents
Bacterial Proteins
Polymethacrylic Acids
triethylene glycol dimethacrylate
Polyethylene Glycols

Grants and funding

The authors received funding only for research project development from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (AUXPE/CAPES/PROEX - 1777/2014) and from Fundação de Amparo à Pesquisa do Estado de São Paulo - (#2012/10750-4). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. http://www.capes.gov.br/. http://www.fapesp.br/