Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya

A K Chakraborty; A Karam; P Mukherjee; L Barkalita; P Borah; S Das; R Sanjukta; K Puro; S Ghatak; I Shakuntala; I Sharma; R G Laha; A Sen

doi:10.1007/s13337-018-0433-9

Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya

Virusdisease. 2018 Mar;29(1):89-95. doi: 10.1007/s13337-018-0433-9. Epub 2018 Feb 17.

Authors

A K Chakraborty^{1

2}, A Karam¹, P Mukherjee^{1

2}, L Barkalita³, P Borah³, S Das¹, R Sanjukta¹, K Puro¹, S Ghatak¹, I Shakuntala¹, I Sharma², R G Laha¹, A Sen¹

Affiliations

¹ Division of Animal Health, ICAR RC for NEH Region, Umiam, Meghalaya India.
² 2Department of Microbiology, Assam University, Silchar, Assam India.
³ Department of Biotechnology, C.V.Sc, AAU, Khanapara, Assam India.

Abstract

The present study focused on the detection and genetic characterisation of 5' untranslated region (5'UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5'UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure.

Keywords: 5′UTR; CSFV; E2; I-TASSER; RT-PCR.