Structural analysis and antioxidant activity of the glycoside from Imperial Chrysanthemum

Bioorg Med Chem Lett. 2018 May 15;28(9):1581-1590. doi: 10.1016/j.bmcl.2018.03.059. Epub 2018 Mar 22.

Abstract

A new glycoside, named as CG-1, was separated from Imperial Chrysanthemum with silica gel column chromatography. The purity was detected by TLC and HPLC. The crystal shape of CG-1 was consisting of a quadrangular and two rectangular pyramids. The analysis of DSC and TGA showed that the melting point of CG-1 crystal was 150.22 °C and had good thermal stability. The monosaccharide conformation analysis showed that it was d-glucose. The structure characteristics were compared by FT-IR, NMR spectroscopy and LC-MS and a molecular structure has been deduced which consistent with spectroscopic results. In vitro antioxidant results suggested that the glycoside extracted from Imperial Chrysanthemum could be effectively employed as natural antioxidant in health or functional food. This work is of significance to keep the antioxidant activity in the processing and application of Imperial Chrysanthemum.

Keywords: Antioxidant; Imperial Chrysanthemum glucoside; Isolation and purification; Molecular structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry*
  • Antioxidants / isolation & purification
  • Chrysanthemum / chemistry*
  • Dose-Response Relationship, Drug
  • Glycosides / chemistry*
  • Glycosides / isolation & purification
  • Molecular Structure
  • Particle Size
  • Structure-Activity Relationship
  • Surface Properties

Substances

  • Antioxidants
  • Glycosides