Rapid identification of carbapenemase-type blaGES and ESBL-type blaGES using multiplex PCR

Shizuo Kayama; Raita Yano; Katsutoshi Yamasaki; Chiemi Fukuda; Keiko Nishimura; Hitoshi Miyamoto; Hiroki Ohge; Motoyuki Sugai

doi:10.1016/j.mimet.2018.03.016

Rapid identification of carbapenemase-type bla_GES and ESBL-type bla_GES using multiplex PCR

J Microbiol Methods. 2018 May:148:117-119. doi: 10.1016/j.mimet.2018.03.016. Epub 2018 Mar 30.

Authors

Shizuo Kayama¹, Raita Yano², Katsutoshi Yamasaki³, Chiemi Fukuda⁴, Keiko Nishimura⁵, Hitoshi Miyamoto⁶, Hiroki Ohge⁷, Motoyuki Sugai⁸

Affiliations

¹ Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8553, Japan; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8553, Japan.
² Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8553, Japan; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8553, Japan; Department of Surgery I, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8553, Japan.
³ Department of Medical Life Science, Kurashiki University of Science and the Arts, Okayama, Japan.
⁴ Kagawa Prefectural Research Institute for Environmental Sciences and Public Health, Kagawa, Japan.
⁵ Department of Clinical Laboratory, Shikoku Medical Center for Children and Adults, Kagawa, Japan.
⁶ Department of Clinical Laboratory, Ehime University Hospital, Ehime, Japan.
⁷ Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8553, Japan; Department of Infectious Diseases, Hiroshima University Hospital, Hiroshima, Japan.
⁸ Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8553, Japan; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8553, Japan. Electronic address: sugai@hiroshima-u.ac.jp.

PMID: 29605523
DOI: 10.1016/j.mimet.2018.03.016

Abstract

Guiana extended-spectrum (GES) β-lactamases are emerging in Japan. The GES family can be classified into 2 groups, one with extended-spectrum β-lactamase (ESBL)-like activity, which hydrolyzes penicillins and cephalosporins, and the other with carbapenemase-like activity with an extended spectrum toward carbapenems. This difference is mediated by variations in a specific amino acid in the GES protein: G170 N or G170S substitutions. We developed an amplification refractory mutation system (ARMS) PCR assay that enabled rapid identification of these variant genes without sequencing.

Keywords: ARMS-PCR; Carbapenemase; bla(GES).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
Gram-Negative Bacteria / enzymology*
Gram-Negative Bacteria / isolation & purification
Gram-Negative Bacterial Infections / microbiology
Humans
Japan
Multiplex Polymerase Chain Reaction / methods*
Time Factors
beta-Lactamases / analysis
beta-Lactamases / classification
beta-Lactamases / genetics*

Substances

beta-Lactamases