CRISPR/Cas13 as a Tool for RNA Interference

Zahir Ali; Ahmed Mahas; Magdy Mahfouz

doi:10.1016/j.tplants.2018.03.003

CRISPR/Cas13 as a Tool for RNA Interference

Trends Plant Sci. 2018 May;23(5):374-378. doi: 10.1016/j.tplants.2018.03.003. Epub 2018 Mar 28.

Authors

Zahir Ali¹, Ahmed Mahas¹, Magdy Mahfouz²

Affiliations

¹ Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
² Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. Electronic address: magdy.mahfouz@kaust.edu.sa.

PMID: 29605099
DOI: 10.1016/j.tplants.2018.03.003

Abstract

Almost all biological processes involve RNA, making it crucial to develop tools for manipulation of the transcriptome. The bacterial CRISPR/Cas13 system was recently rewired to facilitate RNA manipulation in eukaryotes, including plants. We discuss here the opportunities and limitations of using CRISPR/Cas13 in plants for various types of RNA manipulation.

Keywords: CRISPR/Cas13; RNA manipulation; transcriptome regulation; viral interference.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

CRISPR-Cas Systems*
Host-Pathogen Interactions / genetics
Plant Diseases / genetics*
Plant Diseases / virology
Plant Viruses / genetics
Plant Viruses / physiology
Plants / genetics*
Plants / virology
RNA / genetics*
RNA / metabolism
RNA Interference*
RNA, Plant / genetics
RNA, Plant / metabolism
RNA, Viral / genetics
RNA, Viral / metabolism
Technology / methods
Technology / trends

Substances

RNA, Plant
RNA, Viral
RNA