Thiamine is a highly hydrophilic vitamin which is insoluble in common organic solvents, utilized traditionally for synthesis of molecularly imprinted polymer. In this work a simple strategy is introduced to overcome the insolubility problem of thiamine and prepare an efficient MIP in chloroform. Thiamine, as a cationic agent, involved in an ion-pair complex with anionic species of trimethylsilyl-propane sulfonate (TPS). The ion-pair was then transferred effectively to apolar solvent of chloroform where the polymerization reaction was conducted to produce nano-sized thiamine imprinted polymer. The experimental conditions such as pH, ionic strength and carrier agent/thiamine mole ratio were optimized to maximize the thiamine amount, transported to chloroform. The characteristics of the polymer were investigated by FT-IR, SEM and Zeta potential analysis techniques. The experimental adsorption isotherms of thiamine onto MIP and non-imprinted polymer (NIP) were determined and well fitted by Langmuir-Freundlich model. Maximum binding capacities of the MIP and NIP materials were obtained as 106.1 and 35.7 μmol g-1, respectively. After investigation and fixing of the experimental conditions on thiamine extraction by the MIP, it was successfully utilized for thiamine extraction from food samples and its determination by fluorimetric method.
Keywords: Food samples; Hydrophilic compound; Imprinted polymer; Ion-pair; Nanomaterial; Thiamine.
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