Objective: This study aims to investigate the expression of disheveled 2 (Dvl2) around the implant of hyperlipidemic rats at an early stage after the implantation.
Methods: A total of 24 Wistar rats were divided equally into the experimental group fed with high-fat diet group and control group fed with a normal diet. After 8 weeks, the serum lipid levels were detected, and rats received implants in the femur metaphysis. Rats were sacrificed at 1, 3, and 5 days after implantation, and the bones around implants were obtained. Methylene blue-acid fuchsin staining was performed to observe the implant-bone interface. Real-time polymerase chain reaction was performed on runt-related transcription factor 2 (Runx2), cathepsin K (CatK), and Dvl2. Dvl2 Western blot or immunoprecipitation, phosphorylation, and ubiquitination were also conducted.
Results: In the experimental group, less osteoblasts, lower expression of Runx2 and Dvl2, and lower Dvl2 phosphorylation (P<0.05) than those of the control group were observed; furthermore, the CatK expression and Dvl2 ubiquitination were higher than those in the control group (P<0.05).
Conclusions: Hyperlipidemia may suppress bone remodeling around the implant at an early stage by Dvl2 down-regulation, phosphorylation, and up-regulated ubiquitination.
目的 研究蓬乱蛋白2（Dvl2）在高脂血症大鼠种植体周围早期的表达。方法 24只雄性Wistar大鼠，随机均分为实验组和对照组，分别给予高脂和普通饲料。8周后，检测大鼠血脂水平并于股骨干骺端植入种植体，术后1、3、5 d处死，获得种植体周围骨组织。采用亚甲基蓝-酸性品红染色观察种植体-骨界面，采用实时荧光定量聚合酶链反应（RT-PCR）检测Runt相关转录因子2（Runx2）、组织蛋白酶K（CatK）和Dvl2的表达，免疫印迹和免疫共沉淀法检测Dvl2、磷酸化Dvl2和泛素化Dvl2的表达。结果 与对照组相比，实验组的成骨细胞、Runx2、Dvl2和磷酸化Dvl2减少（P<0.05），而破骨细胞、CatK和泛素化Dvl2增多（P<0.05）。结论 高脂血症可能通过上调Dvl2及磷酸化、下调泛素化而抑制种植体周围早期骨改建。.
Keywords: Wnt signal pathway; bone remodeling; disheveled 2; hyperlipidemia.