Glucose oxidase assisted visual detection of glucose using oxygen deficient α-MoO3-x nanoflakes

Mikrochim Acta. 2017 Dec 18;185(1):65. doi: 10.1007/s00604-017-2612-6.

Abstract

An optical method is described for the quantitation of glucose by using oxygen-deficient α-MoO3-x nanoflakes. It is based on the use of glucose oxidase (GOx) which produces hydrogen peroxide on oxidation of glucose. Hydrogen peroxide then oxidizes the α-MoO3-x nanoflakes, and this results in a visible color change from blue to colorless. The color change can be measured photometrically at 740 nm. The method has a 68 nM detection limit. Graphical Abstract Mechanism of glucose detection using blue colored oxygen deficient 2D α-MoO3-x nanoflakes. Hydrogen peroxide (H2O2) is formed as a by-product in the conversion of glucose to glucono-1,5-lactone by glucose oxidase (GOx). In the presence of H2O2, the oxygen vacancies in α-MoO3-x nanoflakes are filled up, and this leads to the loss of blue color of the nanoflakes because they are converted back to colorless bulk α-MoO3.

Keywords: Colorimetry; Hydrogen peroxide; Molybdenum trioxide; Rapid detection; Transition metal oxides; Two dimensional nanoflakes; Visual test.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocatalysis
  • Biosensing Techniques / methods*
  • Color
  • Glucose / analysis*
  • Glucose Oxidase / chemistry
  • Glucose Oxidase / metabolism*
  • Hydrogen Peroxide / metabolism
  • Limit of Detection
  • Models, Molecular
  • Molybdenum / chemistry*
  • Nanostructures / chemistry*
  • Oxidation-Reduction
  • Oxides / chemistry*
  • Oxygen / chemistry*
  • Protein Conformation

Substances

  • Oxides
  • molybdenum trioxide
  • Molybdenum
  • Hydrogen Peroxide
  • Glucose Oxidase
  • Glucose
  • Oxygen