Photoelectrochemical immunoassay for human interleukin 6 based on the use of perovskite-type LaFeO3 nanoparticles on fluorine-doped tin oxide glass

Mikrochim Acta. 2017 Dec 14;185(1):52. doi: 10.1007/s00604-017-2554-z.

Abstract

A film of perovskite-type LaFeO3 nanoparticles (NPs) was deposited on fluorine-doped tin oxide (FTO) conducting glass via dipping-lifting and calcination. Scanning electron microscopy shows that the NPs are evenly distributed on the surface of the glass. The modified glass was further coated with antibody against human interleukin 6 (IL-6) to result in a photoelectrochemical immunosensor for IL-6. The well-established photoelectrochemical immunoassay has a linear current response in the range of 0.1 pg·mL-1 to 0.1 μg·mL-1 and a detection limit as low as 33 fg·mL-1. Graphical abstract Schematic of a novel photoelectochemical immunoassay for the measurement of IL-6 based on perovskite-type LaFeO3 nanoparticles. The immunoassay had a higher sensitivity and may also be applied to other bioanalysis and environment monitoring.

Keywords: Human interleukin-6; Immunoassay; LaFeO3 nanoparticles; Photoelectrochemistry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies
  • Calcium Compounds
  • Electrochemical Techniques
  • Fluorine
  • Humans
  • Immunoassay / methods*
  • Immunoassay / standards
  • Interleukin-6 / analysis*
  • Interleukin-6 / immunology
  • Limit of Detection
  • Oxides / chemistry
  • Photochemical Processes
  • Tin Compounds
  • Titanium

Substances

  • Antibodies
  • Calcium Compounds
  • Interleukin-6
  • Oxides
  • Tin Compounds
  • lanthanum iron oxide
  • perovskite
  • Fluorine
  • Titanium
  • stannic oxide