Fodis: Software for Protein Unfolding Analysis

Nicola Galvanetto; Andrea Perissinotto; Andrea Pedroni; Vincent Torre

doi:10.1016/j.bpj.2018.02.004

Fodis: Software for Protein Unfolding Analysis

Biophys J. 2018 Mar 27;114(6):1264-1266. doi: 10.1016/j.bpj.2018.02.004.

Authors

Nicola Galvanetto¹, Andrea Perissinotto², Andrea Pedroni², Vincent Torre³

Affiliations

¹ International School for Advanced Studies (SISSA), Trieste, Italy. Electronic address: nicola.galvanetto@sissa.it.
² International School for Advanced Studies (SISSA), Trieste, Italy.
³ International School for Advanced Studies (SISSA), Trieste, Italy; Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Zhejiang, People's Republic of China; Center of Systems Medicine, Chinese Academy of Medical Sciences, Suzhou Institute of Systems Medicine, Suzhou Industrial Park, Suzhou, Jiangsu, People's Republic of China.

Abstract

The folding dynamics of proteins at the single-molecule level has been studied with single-molecule force spectroscopy experiments for 20 years, but a common standardized method for the analysis of the collected data and for sharing among the scientific community members is still not available. We have developed a new open-source tool-Fodis-for the analysis of the force-distance curves obtained in single-molecule force spectroscopy experiments, providing almost automatic processing, analysis, and classification of the obtained data. Our method provides also a classification of the possible unfolding pathways and the structural heterogeneity present during the unfolding of proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomechanical Phenomena
Microscopy, Atomic Force*
Protein Unfolding*
Software*