In Vitro Model for Studying of the Role of IGFBP6 Gene in Breast Cancer Metastasizing

Bull Exp Biol Med. 2018 Mar;164(5):688-692. doi: 10.1007/s10517-018-4060-7. Epub 2018 Mar 27.

Abstract

IGFBP6 gene plays an important role in the pathogenesis of breast cancer. In this work, we performed knockdown of IGFBP6 gene in MDA-MB-231 cells and obtained a stable cell line. Knockdown of IGFBP6 gene was confirmed by the real-time PCR. The influence of IGFBP6 gene on migration and proliferation of breast cancer cells was studied. Knockdown of IGFBP6 gene reduced migration activity of MDA-MB-231 cells and increased their proliferation rate. This in vitro cell model can be used for the further analysis of the role of IGFBP6 gene in the pathogenesis of breast cancer.

Keywords: IGFBP6; MDA-MB-231; breast cancer; in vitro model; metastasis spreading.

MeSH terms

  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Movement / physiology
  • Cell Proliferation / genetics
  • Cell Proliferation / physiology
  • Female
  • Gene Expression Regulation, Neoplastic / genetics
  • Humans
  • Insulin-Like Growth Factor Binding Proteins / genetics
  • Insulin-Like Growth Factor Binding Proteins / metabolism*
  • Real-Time Polymerase Chain Reaction

Substances

  • Insulin-Like Growth Factor Binding Proteins
  • insulin-like growth factor binding protein-related protein 1