Synthesis of novel grafted hyaluronic acid with antitumor activity

Abstract

In our study, we aimed to synthesize novel grafted hyaluronic acid with cationic biodegradable polymer, poly (N-vinyl imidazole) (PVI), through free radical polymerization using potassium persulfate as initiator. The effect of various grafting factors including initiator and monomer concentrations, reaction time and temperature was studied on the percentage of grafting parameters such as; graft yield (% GY), grafting efficiency (% GE) and amount of homopolymer formation (% H). Maximum grafted HA was% GY = 235% and%GE = 83% obtained on optimum conditions at [I_n] = 17.5 mmol L^-1, [M] = 1.25 mol L^-1, Temp. = 50 °C, time = 1.5 h and [HA] = 0.025 mol L^-1. The structure of grafted HA (HA-g-PVI) was elucidated via various analysis tools such as; elemental analyses, FTIR, ¹H NMR, XRD, TGA and Field emission scanning electron microscopy (FE-SEM). Hepatic and breast cancers are two common cancer types threatening people worldwide, so, the antitumor activity of two grafted HA samples (% GY = 155% and 235%) was studied against hepatic cancer (HepG-2) and breast cancer cell lines (MCF-7) compared to unmodified HA and PVI. The results showed that antitumor activity of grafted samples was more than unmodified HA and increased with increasing the grafting percentage of PVI onto HA chains, also, the antitumor activity of tested samples against HepG-2 cell lines was higher than MCF-7 cell lines.

Keywords: Antitumor activity; Grafting factors; Grafting parameters; Hyaluronic acid; Poly (N-vinyl imidazole).