Synthesis, characterization and DNA interactions of [Pt3(TPymT)Cl3], the trinuclear platinum(II) complex of the TPymT ligand

Tiziano Marzo; Damiano Cirri; Lorenzo Ciofi; Chiara Gabbiani; Alessandro Feis; Nancy Di Pasquale; Matteo Stefanini; Tarita Biver; Luigi Messori

doi:10.1016/j.jinorgbio.2018.03.009

Synthesis, characterization and DNA interactions of [Pt₃(TPymT)Cl₃], the trinuclear platinum(II) complex of the TPymT ligand

J Inorg Biochem. 2018 Jun:183:101-106. doi: 10.1016/j.jinorgbio.2018.03.009. Epub 2018 Mar 17.

Authors

Tiziano Marzo¹, Damiano Cirri², Lorenzo Ciofi², Chiara Gabbiani³, Alessandro Feis⁴, Nancy Di Pasquale³, Matteo Stefanini⁵, Tarita Biver⁶, Luigi Messori⁷

Affiliations

¹ Department of Chemistry and Industrial Chemistry (DCCI), University of Pisa, Via Moruzzi, 13, 56124 Pisa, Italy; Laboratory of Metals in Medicine (MetMed), Department of Chemistry "U. Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
² Laboratory of Metals in Medicine (MetMed), Department of Chemistry "U. Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
³ Department of Chemistry and Industrial Chemistry (DCCI), University of Pisa, Via Moruzzi, 13, 56124 Pisa, Italy.
⁴ Department of Chemistry "U. Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
⁵ DI.V.A.L Toscana S.R.L., Via Madonna del Piano, 6, 50019 Sesto Fiorentino, Italy.
⁶ Department of Chemistry and Industrial Chemistry (DCCI), University of Pisa, Via Moruzzi, 13, 56124 Pisa, Italy. Electronic address: tarita.biver@unipi.it.
⁷ Laboratory of Metals in Medicine (MetMed), Department of Chemistry "U. Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy. Electronic address: luigi.messori@unifi.it.

PMID: 29576241
DOI: 10.1016/j.jinorgbio.2018.03.009

Abstract

The triplatinum complex of the 2,4,6-Tris(2-pyrimidyl)-1,3,5-triazine ligand, Pt₃TPymT hereafter, has been prepared and characterized for the first time. NMR studies point out that the three platinum(II) centers possess an identical coordination environment. The interactions of Pt₃TPymT with DNA were explored in comparison to the free ligand. Specifically, fluorescence, mass spectrometry, viscometry and melting measurements were carried out. In contrast to expectations, the obtained data reveal that no intercalative binding takes place; we propose that binding of Pt₃TPymT to DNA mainly occurs through external/groove binding.

Keywords: Anticancer drug; Coordination chemistry; DNA; Platinum; TPymT.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents / chemistry*
Coordination Complexes / chemistry*
Magnetic Resonance Spectroscopy
Mass Spectrometry
Organoplatinum Compounds / chemistry*
Platinum / chemistry*

Substances

Antineoplastic Agents
Coordination Complexes
Organoplatinum Compounds
Platinum