The performance of one-step nucleic acid amplification assay for intraoperative detection of sentinel lymph node macrometastasis in breast cancer: An updated meta-analysis

Fang Shi; Zhenzhen Liang; Qian Zhang; Chunpeng Wang; Xin Liu

doi:10.1016/j.breast.2018.03.005

The performance of one-step nucleic acid amplification assay for intraoperative detection of sentinel lymph node macrometastasis in breast cancer: An updated meta-analysis

Breast. 2018 Jun:39:39-45. doi: 10.1016/j.breast.2018.03.005. Epub 2018 Mar 22.

Authors

Fang Shi¹, Zhenzhen Liang², Qian Zhang³, Chunpeng Wang⁴, Xin Liu⁵

Affiliations

¹ Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: shifang16@mails.jlu.edu.cn.
² Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: liangzz15@mails.jlu.edu.cn.
³ Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: zhangqian15@mails.jlu.edu.cn.
⁴ School of Mathematics and Statistics, Northeast Normal University, Changchun, Jilin, China. Electronic address: wangcpnenu@163.com.
⁵ Department of Epidemiology and Statistics, School of Public Health, Jilin University, Changchun 130021, China. Electronic address: liuxinjlu01@163.com.

PMID: 29574393
DOI: 10.1016/j.breast.2018.03.005

Abstract

Background: One-step nucleic acid amplification (OSNA) assay is a molecular diagnostic method for intraoperative detection of lymph node metastasis by amplifying cytokeratin 19 mRNA. We aim to further evaluate the performance of OSNA assay for the intraoperative diagnosis of sentinel lymph node metastasis in breast cancer.

Methods: PubMed, Cochrane Library, Web of Science databases were searched to retrieve related literature published up until December 2017. This meta-analysis was performed using a random-effects model. Risks of bias and quality assessments of included studies were evaluated and subgroup analysis was performed.

Results: Nineteen studies were included in this meta-analysis. For overall metastasis, the pooled sensitivity, specificity and area under the summary receiver-operating characteristic curve (AUC) were 0.90, 0.96 and 0.98, respectively. For macrometastasis, the pooled sensitivity, specificity and AUC were 0.85, 0.98 and 0.94, respectively.

Conclusion: OSNA assay is an accurate molecular diagnosis for intraoperative detection of sentinel lymph node macrometastasis in breast cancer.

Keywords: Breast cancer; Diagnosis; Meta-analysis; Metastasis; One-step nucleic acid amplification; Sentinel lymph node.

Publication types

Meta-Analysis
Review

MeSH terms

Area Under Curve
Breast Neoplasms / genetics
Breast Neoplasms / pathology
Breast Neoplasms / surgery*
Female
Humans
Intraoperative Care / methods*
Keratin-19 / analysis
Lymphatic Metastasis / diagnosis*
Lymphatic Metastasis / pathology
Middle Aged
Molecular Diagnostic Techniques / methods
Molecular Diagnostic Techniques / statistics & numerical data*
Nucleic Acid Amplification Techniques / methods
Nucleic Acid Amplification Techniques / statistics & numerical data*
RNA, Messenger / analysis
ROC Curve
Sensitivity and Specificity
Sentinel Lymph Node / pathology
Sentinel Lymph Node / surgery

Substances

Keratin-19
RNA, Messenger