Tetramer-based identification of naïve antigen-specific B cells within a polyclonal repertoire

Jeremy F Brooks; Xiao Liu; Janet M Davies; James W Wells; Raymond J Steptoe

doi:10.1002/eji.201747447

Tetramer-based identification of naïve antigen-specific B cells within a polyclonal repertoire

Eur J Immunol. 2018 Jul;48(7):1251-1254. doi: 10.1002/eji.201747447. Epub 2018 Apr 17.

Authors

Jeremy F Brooks¹, Xiao Liu¹, Janet M Davies², James W Wells¹, Raymond J Steptoe¹

Affiliations

¹ The University of Queensland, Translational Research Institute, University of Queensland Diamantina Institute, Brisbane, Australia.
² School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.

PMID: 29572817
DOI: 10.1002/eji.201747447

Abstract

Detecting naïve antigen-specific B cells can be challenging. Use of multiple, complementary tetramers with different fluorochromes enhances sensitivity and specificity allowing naïve antigen-specific B cells to be readily distinguished within a polyclonal repertoire. Activated, affinity-matured B cells, however, can be detected effectively using a single tetramer.

Keywords: B cells; Flow cytometry; Tetramer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibody Affinity
Antigens / metabolism
B-Lymphocyte Subsets / immunology*
B-Lymphocytes / immunology*
Cells, Cultured
Epitopes
Flow Cytometry / methods*
Fluorescent Dyes / metabolism
HLA Antigens / metabolism
Humans
Lymphocyte Activation
Protein Binding
Receptors, Antigen, B-Cell / genetics
Receptors, Antigen, B-Cell / immunology*
Sensitivity and Specificity

Substances

Antigens
Epitopes
Fluorescent Dyes
HLA Antigens
Receptors, Antigen, B-Cell