Functionalized poly(pyrrole-3-carboxylic acid) nanoneedles for dual-imaging guided PDT/PTT combination therapy

Xin Liu; Huiling Su; Wei Shi; Yang Liu; Yanan Sun; Dongtao Ge

doi:10.1016/j.biomaterials.2018.03.030

Functionalized poly(pyrrole-3-carboxylic acid) nanoneedles for dual-imaging guided PDT/PTT combination therapy

Biomaterials. 2018 Jun:167:177-190. doi: 10.1016/j.biomaterials.2018.03.030. Epub 2018 Mar 16.

Authors

Xin Liu¹, Huiling Su¹, Wei Shi², Yang Liu¹, Yanan Sun¹, Dongtao Ge³

Affiliations

¹ Key Laboratory of Biomedical Engineering of Fujian Province University/Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China.
² Key Laboratory of Biomedical Engineering of Fujian Province University/Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China. Electronic address: shiwei@xmu.edu.cn.
³ Key Laboratory of Biomedical Engineering of Fujian Province University/Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China. Electronic address: gedt@xmu.edu.cn.

PMID: 29571053
DOI: 10.1016/j.biomaterials.2018.03.030

Abstract

Herein, poly(pyrrole-3-carboxylic acid) (PPyCOOH) nanoneedles with abundant carboxyl groups were synthesized by aqueous dispersion polymerization method using pyrrole-3-carboxylic acid as conductive polymer monomer. The PPyCOOH nanoneedles not only owned good photothermal performance, but also more importantly showed enhanced tumor cell uptake efficiency (1.64 fold) compared with size and zeta-potential matched nanospheres. After loading photosensitizer aluminum phthalocyanine tetrasulfonate (AlPCS₄) and modifying with poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) onto the PPyCOOH nanoneedles, novel nanoneedle complexes (AlPCS₄@PPyCONH-PAH-PAA) integrating photodynamic therapy (PDT) and photothermal therapy (PTT) were successfully fabricated. The as-prepared nanoneedle complexes improved uptake efficiency of AlPCS₄ both in vitro and in vivo. Moreover, the nanoneedle complexes have infrared thermal and fluorescent properties. By combined PDT/PTT under the guiding of dual modal imaging, the tumors in mice were completely eliminated and no recurrence was observed in 30 days after treatment, indicating that PPyCOOH nanoneedles have great potential as a novel drug carrier for constructing multifunctional nanoplatform used for cancer treatment.

Keywords: Dual-imaging guided; Nanoneedle; Photodynamic therapy; Photothermal therapy; Poly(pyrrole-3-carboxylic acid).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carboxylic Acids / therapeutic use*
Combined Modality Therapy / methods
Female
HeLa Cells
Humans
Hyperthermia, Induced / methods
Indoles / therapeutic use*
Mice
Mice, Inbred BALB C
Mice, Nude
Nanostructures / therapeutic use*
Neoplasms / diagnostic imaging
Neoplasms / therapy*
Optical Imaging / methods
Organometallic Compounds / therapeutic use*
Photochemotherapy / methods
Photosensitizing Agents / therapeutic use*
Polymers / therapeutic use*
Pyrroles / therapeutic use*
RAW 264.7 Cells
Theranostic Nanomedicine / methods*
Thermography / methods

Substances

Carboxylic Acids
Indoles
Organometallic Compounds
Photosensitizing Agents
Polymers
Pyrroles
poly(pyrrole-3-carboxylic acid)
aluminum tetrasulfophthalocyanine