Abstract
Oligodendrocytes (OLs) are myelinating glial cells that form myelin sheaths around axons to ensure rapid and focal conduction of action potentials. Here, we found that an axonal outgrowth regulatory molecule, AATYK (apoptosis-associated tyrosine kinase), was up-regulated with OL differentiation and remyelination. We therefore studied its role in OL differentiation. The results showed that AATYK knockdown inhibited OL differentiation and the expression of myelin genes in vitro. Moreover, AATYK-deficiency maintained the proliferation status of OLs but did not affect their survival. Thus, AATYK is essential for the differentiation of OLs.
Keywords:
AATYK; Differentiation; Oligodendrocytes.
MeSH terms
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Animals
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Animals, Newborn
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Apoptosis Regulatory Proteins / genetics
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Apoptosis Regulatory Proteins / metabolism*
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Cell Differentiation / drug effects
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Cell Differentiation / physiology*
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Cell Proliferation / drug effects
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Cell Proliferation / genetics
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Cells, Cultured
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Cuprizone / toxicity
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Demyelinating Diseases / chemically induced
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Demyelinating Diseases / metabolism
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Demyelinating Diseases / pathology
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Embryo, Mammalian
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Gene Expression Regulation, Developmental / genetics*
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Ki-67 Antigen / metabolism
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Mice
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Mice, Inbred C57BL
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Myelin Basic Protein / metabolism
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Myelin Proteolipid Protein / metabolism
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Myelin Sheath / drug effects
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Myelin Sheath / metabolism*
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Oligodendroglia / drug effects
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Oligodendroglia / metabolism*
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / metabolism*
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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Rats
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Rats, Sprague-Dawley
Substances
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Apoptosis Regulatory Proteins
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Ki-67 Antigen
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Myelin Basic Protein
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Myelin Proteolipid Protein
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Plp1 protein, mouse
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RNA, Small Interfering
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Cuprizone
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Aatk protein, mouse
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Protein-Tyrosine Kinases