Extracellular Application of CRMP2 Increases Cytoplasmic Calcium through NMDA Receptors

Neuroscience. 2018 Apr 15:376:204-223. doi: 10.1016/j.neuroscience.2018.02.002.

Abstract

Collapsin Response Mediator Protein 2 (CRMP2) is an intracellular protein involved in axon and dendrite growth and specification. In this study, CRMP2 was identified in a conditioned media derived from degenerated sciatic nerves (CM). On cultured rat hippocampal neurons, acute extracellular application of CM or partially purified recombinant CRMP2 produced an increase in cytoplasmic calcium. The increase in cytoplasmic calcium was mostly mediated through NMDA receptors, with a minor contribution of N-type VDCC, and it was maintained as long as CM was present. By using live-labeling of CRMP2, Ca2+ channel binding domain 3 (CBD3) peptide derived from CRMP2, and recombinant CRMP2, we demonstrated that that this effect was mediated by an action on the extracellular side of the NMDA receptor. This is the first report of an extracellular action of CRMP2. Prolonged exposure to extracellular CRMP2, may contribute to neuronal calcium dysregulation and neuronal damage.

Keywords: CBD3 peptide; calcium dysregulation confocal; calcium imaging; excitotoxicity; hippocampal neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Cations, Divalent / metabolism
  • Cells, Cultured
  • Central Nervous System Agents / administration & dosage*
  • Central Nervous System Agents / isolation & purification
  • Culture Media, Conditioned
  • Cytoplasm / drug effects*
  • Cytoplasm / metabolism
  • Extracellular Space
  • Hippocampus / drug effects
  • Hippocampus / metabolism
  • Intercellular Signaling Peptides and Proteins / administration & dosage*
  • Male
  • Mice
  • Molecular Docking Simulation
  • Nerve Tissue Proteins / administration & dosage*
  • Nerve Tissue Proteins / isolation & purification
  • Nerve Tissue Proteins / metabolism
  • Neurons / drug effects
  • Neurons / metabolism
  • Optic Nerve / metabolism
  • Rats, Sprague-Dawley
  • Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Recombinant Proteins / administration & dosage
  • Sciatic Nerve / metabolism

Substances

  • Cations, Divalent
  • Central Nervous System Agents
  • Culture Media, Conditioned
  • Intercellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • Receptors, N-Methyl-D-Aspartate
  • Recombinant Proteins
  • collapsin response mediator protein-2
  • Calcium