Multipotent mesenchymal stromal cells (MMSCs) are essential for regeneration and tissue homeostasis. The investigation was focused on the functional state of MMSCs isolated from human adipose tissue and exposed to simulated microgravity in vitro by RPM (random position machine). The results point to an increase of proliferative potential, decrease of the lisosomal compartment activity, and reductions in cell size and granularity. Evaluation of the paracrine activity in relatively static control revealed increased IL-8 production and decreased IL-6 production. *Our .data supplement previous findings and allow conclude about universality of the mesenchymal cell reaction to simulated microgravity. The investigation did not detect signs of cell stress in this environment.