Mammalian orthoreovirus (reovirus) utilizes pore forming peptides to penetrate host cell membranes. This step is essential for delivering its genome containing core particle during viral entry. This protocol describes an in vitro assay for measuring reovirus-induced pore formation.
Keywords: Hemolysis; Mammalian orthoreovirus; Membrane penetration; Reoviridae; Viral entry; Virology.