Propylene glycol guluronate sulfate (PGGS) reduces lipid accumulation via AMP-activated kinase activation in palmitate-induced HepG2 cells

Meng Xin; Yang Sun; Haijiao Chen; Quancai Li; Yunlou Dun; Huashi Guan; Jiejie Hao; Chunxia Li

doi:10.1016/j.ijbiomac.2018.03.068

Propylene glycol guluronate sulfate (PGGS) reduces lipid accumulation via AMP-activated kinase activation in palmitate-induced HepG2 cells

Int J Biol Macromol. 2018 Jul 15:114:26-34. doi: 10.1016/j.ijbiomac.2018.03.068. Epub 2018 Mar 15.

Authors

Meng Xin¹, Yang Sun¹, Haijiao Chen¹, Quancai Li¹, Yunlou Dun¹, Huashi Guan², Jiejie Hao³, Chunxia Li⁴

Affiliations

¹ Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
² Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
³ Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. Electronic address: 2009Haojie@ouc.edu.cn.
⁴ Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. Electronic address: lchunxia@ouc.edu.cn.

PMID: 29550423
DOI: 10.1016/j.ijbiomac.2018.03.068

Abstract

Cardiovascular disease (CVD) is the No. 1 cause of death worldwide. Hyperlipidemia is one of the major risk factors for CVD. Maintaining lipid homeostasis is an effective way to prevent CVD. We prepared propylene glycol guluronate sulfate (PGGS), a sulfated polysaccharide, and investigated its effect on lipid metabolism in HepG2 cells. We found that total cholesterol (TC) and triglycerides (TG) were significantly decreased in the cells after PGGS treatment. We have also shown that the AMPK signaling is activated after PGGS treatment as evidenced by changes in the expression of many AMPK downstream targets including SREBP-1c, SIRT-1, CPT1, PPARα, and FAS. Our results have demonstrated that PGGS is a potentially novel lipid-lowering agent for CVD prevention.

Keywords: AMPK; HepG2; Hyperlipidemia; Sulfated polysaccharides; Total cholesterol; Triglycerides.

MeSH terms

AMP-Activated Protein Kinases / metabolism*
Cholesterol / biosynthesis*
Hep G2 Cells
Humans
Lipid Metabolism / drug effects*
Palmitic Acid / toxicity*
Propylene Glycol / pharmacology*
Triglycerides / biosynthesis*

Substances

Triglycerides
Palmitic Acid
Propylene Glycol
Cholesterol
AMP-Activated Protein Kinases