Comparison of ATP sulfurylase 2 from selenium hyperaccumulator Stanleya pinnata and non-accumulator Stanleya elata reveals differential intracellular localization and enzyme activity levels

Ying Jiang; Michela Schiavon; Leonardo W Lima; Tripti; Rachel R Jones; Ali F El Mehdawi; Suzanne Royer; Zhaohai Zeng; Yuegao Hu; Elizabeth A H Pilon-Smits; Marinus Pilon

doi:10.1016/j.bbagen.2018.03.014

Comparison of ATP sulfurylase 2 from selenium hyperaccumulator Stanleya pinnata and non-accumulator Stanleya elata reveals differential intracellular localization and enzyme activity levels

Biochim Biophys Acta Gen Subj. 2018 Nov;1862(11):2363-2371. doi: 10.1016/j.bbagen.2018.03.014. Epub 2018 Mar 14.

Authors

Affiliations

¹ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
² Department of Biology, Colorado State University, Fort Collins, CO 80523, USA; DAFNAE Department, Padova University, Agripolis, 35020, Legnaro, Padua, Italy.
³ Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
⁴ Department of Biology, Colorado State University, Fort Collins, CO 80523, USA; Department of Experimental Biology and Biotechnology, Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620002, Russia.
⁵ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
⁶ Department of Biology, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: pilon@colostate.edu.

PMID: 29548763
DOI: 10.1016/j.bbagen.2018.03.014

Abstract

Background: The plant Stanleya pinnata hyperaccumulates Se up to 0.5% of its dry weight in organic forms, whereas the closely related Stanleya elata does not hyperaccumulate Se. ATP sulfurylase (ATPS) can catalyze the formation of adenosine 5'-phosphoselenate (APSe) from ATP and selenate. We investigated the S. pinnata ATPS2 isoform (SpATPS2) to assess its possible role in Se hyperaccumulation.

Methods: ATPS expression and activity was compared in the two Stanleya species. The ATPS2 protein sequences were modeled. Sub-cellular locations were analyzed using GFP fusions. Enzyme activity of purified recombinant SpATPS2 was measured.

Results: ATPS2 transcript levels were six-fold higher in roots of S. pinnata relative to S. elata. Overall root ATPS enzyme activity was two-fold elevated in S. pinnata. Cloning and sequencing of SpATPS2 and S. elata ATPS2 (SeATPS2) showed the predicted SeATPS2 to be canonical, while SpATPS2, although very similar in its core structure, has unique features, including an interrupted plastid targeting signal due to a stop codon in the 5' region of the coding sequence. Indeed GFP fusions revealed that SpATPS2 had exclusive cytosolic localization, while SeATPS2 showed dual localization in plastids and cytosol. SpATPS2 activity was inhibited by both sulfate and selenate, indicating that the enzyme acts on both substrates.

Conclusions: The ATPS2 from S. pinnata differs from non-accumulator ATPS2 in its elevated expression and sub-cellular localization. It likely acts on both selente and sulfate substrates.

General significance: These observations shed new light on the role of ATPS2 in the evolution of Se hyperaccumulation in plants. This article is part of a Special Issue entitled Selenium research in biochemistry and biophysics - 200 year anniversary issue, edited by Dr. Elias Arnér and Dr. Regina Brigelius-Flohe.

Keywords: ATP sulfurylase; Hyperaccumulation; Selenium; Stanleya pinnata.