Yak milk casein as potential precursor of angiotensin I-converting enzyme inhibitory peptides based on in silico proteolysis

Kai Lin; Lan-Wei Zhang; Xue Han; Liang Xin; Zhao-Xu Meng; Pi-Min Gong; Da-You Cheng

doi:10.1016/j.foodchem.2018.02.051

Yak milk casein as potential precursor of angiotensin I-converting enzyme inhibitory peptides based on in silico proteolysis

Food Chem. 2018 Jul 15:254:340-347. doi: 10.1016/j.foodchem.2018.02.051. Epub 2018 Feb 10.

Authors

Kai Lin¹, Lan-Wei Zhang², Xue Han³, Liang Xin⁴, Zhao-Xu Meng⁵, Pi-Min Gong⁶, Da-You Cheng⁷

Affiliations

¹ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China. Electronic address: linkai@hit.edu.cn.
² School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Hua Ling Biotechnology Research Center, Lanzhou 730000, China. Electronic address: zhanglw@hit.edu.cn.
³ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China. Electronic address: xhan@hit.edu.cn.
⁴ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China. Electronic address: xinliang@hit.edu.cn.
⁵ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China. Electronic address: mengzhaoxu@hit.edu.cn.
⁶ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China. Electronic address: 14b922007@hit.edu.cn.
⁷ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China. Electronic address: tycdy@hit.edu.cn.

PMID: 29548462
DOI: 10.1016/j.foodchem.2018.02.051

Abstract

Yak milk casein was selected as a potential precursor of bioactive peptides based on in silico analysis. Most notable among these are the angiotensin I-converting enzyme (ACE) inhibitory peptides. First, yak milk casein has high homology with cow milk casein by homologous analysis. The potential of yak milk casein for the releasing bioactive peptides was evaluated by determining the frequency of occurrence of fragments with a given activity. Through the BIOPEP database analysis, there are many bioactive peptides in yak milk casein sequences. Then, an in silico proteolysis using single or combined enzymes to obtained ACE inhibitory peptides was investigated. Cytotoxicity analysis using the online toxic prediction tool ToxinPred revealed that all in silico proteolysis derived ACE inhibitory peptides are non-cytotoxic. Overall, the present study highlights a in silico proteolysis approach to assist the yak milk casein releasing ACE inhibitory peptides and provides a guidance for the actual hydrolysis of proteins for the production of bioactive peptides.

Keywords: Angiotensin I-converting enzyme inhibitory peptide; Bioactive peptides; In silico proteolysis; Yak milk casein.

MeSH terms

Angiotensin-Converting Enzyme Inhibitors / chemistry
Angiotensin-Converting Enzyme Inhibitors / pharmacology*
Angiotensin-Converting Enzyme Inhibitors / toxicity
Animals
Caseins / chemistry*
Cattle
Computer Simulation
Female
Milk / chemistry*
Peptides / chemistry
Peptides / pharmacology*
Peptides / toxicity
Proteolysis

Substances

Angiotensin-Converting Enzyme Inhibitors
Caseins
Peptides