Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve

Rebecca L Cunningham; Kelly R Monk

doi:10.1007/978-1-4939-7649-2_26

Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve

Methods Mol Biol. 2018:1739:385-400. doi: 10.1007/978-1-4939-7649-2_26.

Authors

Rebecca L Cunningham¹, Kelly R Monk^{2

3}

Affiliations

¹ Department of Developmental Biology, Washington University in St. Louis, St. Louis, MO, USA.
² Vollum Institute, Oregon Health & Science University, Portland, OR, USA. monk@ohsu.edu.
³ Department of Developmental Biology, Washington University in St. Louis, St. Louis, MO, USA. monk@ohsu.edu.

PMID: 29546722
DOI: 10.1007/978-1-4939-7649-2_26

Abstract

Transmission electron microscopy (TEM) enables visualization of the ultrastructure of the myelin sheath. Schwann cells on the posterior lateral line nerves and motor nerves can be imaged by TEM. Here, we detail the multiday processing of larval trunks and dissected posterior lateral line for TEM, as well as how to trim embedded samples, section, and stain grids for imaging.

Keywords: Adult zebrafish lateral line nerve; Lateral line nerve; Motor nerves; Myelin ultrastructure; Transmission electron microscopy.

MeSH terms

Animals
Larva / cytology
Larva / ultrastructure
Microscopy, Electron, Transmission / methods*
Myelin Sheath / metabolism
Schwann Cells / cytology
Schwann Cells / ultrastructure
Zebrafish